MSI99P0114 | |
Autrey, L. J. C. Saumtally, S. Dookun, A. Boolell, S. | |
Occurrence of sugar cane bacilliform virus in Mauritius | |
Periodical article | |
1990 | |
Proc. S. Afr. Sug. Technol. Ass. | |
64: 34-39 | |
En | |
En | |
Sugarcane bacilliform virus (SCVB) has been observed for the first time in noble canes and interspecific hybrids in Mauritius. The latter included commercial varieties such as M438/59, M555/60, S17 and B51129 as well as some clones imported from Barbados, India, USA, Taiwan, South Africa and Australia. A range of symptoms, consisting of mild freekling to severe chlorosis with a mosaic pattern was observed, particularly in noble canes. In interspecific hybrids the symptoms were less severe. The virus was diagnosed by DAS ELISA, only in crude sap from some noble canes such as Iscambine striped and Tanna white with severe symptoms. This method was however unsatisfactory, although it is used routinely in Morocco. Similarly, direct examinations of crude sap by the quick dip technique in the electron microscope proved unreliable, except for a few highly infected noble canes. Virus particles were readily detected by immune electron microscopy using an antiserum to a Moroccan isolate of SCBV. The virus was purified by differential centrifugation and separation over 10-40 per cent sucrose CsCl gradients. The dimensions of particles were 120-140 x 25-30 nm both in crude sap extracts and purified preparations. Various heat treatments used for controlling sugarcane diseases failed to free infected cuttings from SCBV. It is inferred that there might be some variation in SCBV resulting in different properties between the disease in Mauritius and Morocco. The need to identify the vector of SCBV, to establish its economic importance and its world distribution and to determine the relationship between bacilliform viruses found elsewhere insugarcane is stressed. Emphasis is laid on the importance of an international collaborative project to prevent further spread of the disease. | |
SUGARCANE PLANT VIRUSES DISEASES SCBV SUGAR CANE BACILLIFORM VIRUS | |
MAURITIUS | |
Sugarcane: Diseases and disease management | |
Viruses: SCBV | |
1992-02-07 | |
En | |
Mauritius Sugar Industry Research Institute | |
LIB | |
CAT | |
1 | |
PATH |