MSI02P3711 | |
Jaufeerally-Fakim, Y. Autrey, L. J. C. Toth, I. Daniels, M. Dookun, A. | |
Amplification polymorphism among Xanthomonas albilineans strains, using a single oligonucleotide primer | |
Periodical article | |
2002 | |
European J. Pl. Path. | |
108: 121-130 | |
En | |
En | |
A genomic library was produced by a subtractive hybridisation method using DNA from Xanthomonas albilineans seroval I and X. albilineans serovar II, originating from Mauritius. The cloned fragments were amplified and used as probes for Southern hybridisation. Probe F20, strains of serovars I and II were differentiated by their banding profiles. This probe was sequenced and oligonucleotide primers were designed. Fragment number and length polymorphisms were obtained after PCR amplification of X. albilineans DNA using F20A as a single primer. The number and size of bands obtained with this primer was correlated to the serotypes of the strains and to the DNA grouping reported by Alvarez et al. (1996). The two serotypes I and II which exist in Mauritius were differentiated by the PCR using primer F20A. The probe and primer developed provide rapid and precise tools for the differentiation of genetic variants within the species X. albilineans. | |
Xanthomonas albilineans DNA probes Oligonucleotide primer Amplification polymorphism Leaf scald diseases | |
Mauritius | |
Sugarcane: Diseases and disease management | |
Leaf scald | |
06-06-2002 | |
En | |
LIB | |
CAT | |
BIOTECH |