Antigenic differences in Xanthomonas albilineans, causal agent of leaf scald disease of sugarcane
Code (CO)MSI99P1448
Author (AU)Dookun, A.
Saumtally, S.
Autrey, L. J. C.
Title - English (ET)Antigenic differences in Xanthomonas albilineans, causal agent of leaf scald disease of sugarcane
Thesis-Parent title (TH)Lemattre, M.; Freigoun, S.; Rudolph, K.; Swings, J. G. (eds), Plant pathogenic bacteria, 8. International Congress, Versailles (France), June 9-12, 1992.
Document Type(DT)Conference paper
Date of publication (DP)1994
Series (SE)Colloques de l'INRA, no. 66
Source (SO)p. 301-310
Publisher (PB)Paris, Institut National de la Recherche Agronomique (INRA) and Institut Français de Recherche Scientifique pour le Développement Agronomique
Language of text (LT)En
Language of summaries (LS)En
Abstract (AB)Two serotypes of Xanthomonas albilineans namely the Mascarene and African ones have been identified in Mauritius. The Mascarene one was more frequenntly encountered and 84 per cent of the isolates characterized belonged to this serotype. Isolates from both serotypes produced similar symptoms in the field and had the same cultural characteristics, biochemical and physiological properties. Polyclonal antisera produced in rabbits were specific to each serotype in Ouchterlony's double diffusion test when using heat fixed bacterial cells as antigens. Protein profiles by polyacrylamide gel electrophoresis and fatty acid analyses of isolates did not reveal any differences between the two serotypes. However, different banding patterns were observed when the lipopolysaccharides (LPS) extracts from cultures of the two serotypes were compared on polyacrylamide gels. Transfer of the LPS to nitrocellulose membranes and probing with antisera showed that the LPS from each serotype reacted specifically with its homologous antiserum. It seems that the LPS is the antigenic component responsible for serotypic differences and this could prove useful in the production of monoclonal antibodies to each serotype.
Descriptors - English (DE)SUGARCANE
XANTHOMONAS ALBILINEANS
ANTIGENS
DISEASES
BACTERIAL DISEASES
LEAF SCALD
Descriptors - Geographic (DG)MAURITIUS
Sort Key 1(K1)Sugarcane: Diseases and disease management
Date record entered (DA)1993-01-08
Language of analysis (LA)En
Location (LO)LIB
Processing status (PS)CAT
Number of copies (NC)1
MSIRI Staff (MS)PATH
BIOTECH