Amplification polymorphism among Xanthomonas albilineans strains, using a single oligonucleotide primer
Code (CO)MSI02P3711
Author (AU)Jaufeerally-Fakim, Y.
Autrey, L. J. C.
Toth, I.
Daniels, M.
Dookun, A.
Title - English (ET)Amplification polymorphism among Xanthomonas albilineans strains, using a single oligonucleotide primer
Document Type(DT)Periodical article
Date of publication (DP)2002
Series (SE)European J. Pl. Path.
Source (SO)108: 121-130
Language of text (LT)En
Language of summaries (LS)En
Abstract (AB)A genomic library was produced by a subtractive hybridisation method using DNA from Xanthomonas albilineans seroval I and X. albilineans serovar II, originating from Mauritius. The cloned fragments were amplified and used as probes for Southern hybridisation. Probe F20, strains of serovars I and II were differentiated by their banding profiles. This probe was sequenced and oligonucleotide primers were designed. Fragment number and length polymorphisms were obtained after PCR amplification of X. albilineans DNA using F20A as a single primer. The number and size of bands obtained with this primer was correlated to the serotypes of the strains and to the DNA grouping reported by Alvarez et al. (1996). The two serotypes I and II which exist in Mauritius were differentiated by the PCR using primer F20A. The probe and primer developed provide rapid and precise tools for the differentiation of genetic variants within the species X. albilineans.
Descriptors - English (DE)Xanthomonas albilineans
DNA probes
Oligonucleotide primer
Amplification polymorphism
Leaf scald
diseases
Descriptors - Geographic (DG)Mauritius
Sort Key 1(K1)Sugarcane: Diseases and disease management
Sort Key 2 (K2)Leaf scald
Date record entered (DA)06-06-2002
Language of analysis (LA)En
Location (LO)LIB
Processing status (PS)CAT
MSIRI Staff (MS)BIOTECH