Constraints in sugarcane micropropagation by tissue culture
| MSI99P2034 | |
| Dookun, A. Moutia, M. Mulleegadoo, K. Autrey, L. J. C. | |
| Constraints in sugarcane micropropagation by tissue culture | |
| Periodical article | |
| 1995 | |
| International Sugar Journal (Supplement) | |
| 97(1161): A27-A28 | |
| Poster presented at the XXII ISSCT Congress, Cartagena, Colombia, 5-13 September 1995. Abstract published in International Sugar Journal (Supplement), September 1995 | |
| En | |
| En | |
| Contamination was a severe problem when sugar cane auxiliary buds were used to initiate plantlets for in vitro multiplication. The contaminants were saprophytic bacteria belonging mainly to the Enterobacter and Bacillus spp. The use of hot water treatments of cuttings prior to excision of buds did not reduce the level of contamination. Disinfection with sodium hypochlorite for 20 min at a concentration of 1.7 per cent gave the best result for surface sterilization. Cultures were contaminated soon after splitting and subculturing. Problems were also encountered during the acclimatization period in the glasshouse or after transplantation in the field. Plantlet blight caused by fungi was adequately controlled by the application of iprodione while foliar sprays of iron chelate helped to revert chlorotic symptoms due to iron deficiency. Adequate water supply during the early stage of transplantation to the field was found to be essential for plantlet establishment. The tissue culture process did not eliminate sugar cane diseases such as leaf scald and sugar cane bacilliform virus. | |
| SUGARCANE MICROPROPAGATION TISSUE CULTURE | |
| MAURITIUS | |
| Cane breeding and genetics | |
| Breeding: Methods: Tissue culture | |
| 1995-09-29 | |
| En | |
| LIB | |
| CAT | |
| BIOTECH |