Cultural characteristics of Mycovellosiella koepkei (Kruger) Deighton on various media and their relationship with varietal reaction on cane leaf agar
| MSI99P0214 | |
| Autrey, L. J. C. Saumtally, S. | |
| Cultural characteristics of Mycovellosiella koepkei (Kruger) Deighton on various media and their relationship with varietal reaction on cane leaf agar | |
| Periodical article | |
| 1986 | |
| Proc. int. Soc. Sug. Cane Technol. | |
| 19: 336-343 | |
| En | |
| En | |
| Six methods were investigated to isolate the fungus Mycovellosiella koepkei the causal agent of yellow spot disease. The most successful one consisted in transferring spores from field collected material or from leaves left to sporulate in a humid chamber to agar plates, using a sterile fine needle, and then streaking them by means of a platinum wire loop. Little contamination was occasioned by this method in comparison with the other ones used. Among five media, Czapek yeast extract was the best for isolation but cane leaf agar (CLA) was more appropriate for maintenance of cultures as growth was slightly faster and sporulation much better on CLA than on the other media. Colony morphology was the same on all media but the length of conidia varied, being greatest on CLA and least on ca rrot decoction agar. In pure culture the conidia were shorter than on infected leaves. The fungus grew faster and sporulated better when CLA was prepared from susceptible sugarcane varieties rather than from resistant ones. In vitro culture on CLA could provide an interesting method of screening for varietal resistance to yellow spot disease. | |
| SUGARCANE FUNGI MYCOVELLOSIELLA KOEPKEI FUNGAL DISEASES DISEASES YELLOW SPOT | |
| MAURITIUS | |
| Sugarcane: Diseases and disease management | |
| Fungal diseases: Yellow spot | |
| 1992-02-15 | |
| En | |
| Mauritius Sugar Industry Research Institute | |
| LIB | |
| CAT | |
| PATH |