Detection and characterization of phytoplasmas from sugar cane and weeds
| MSI06P4225 | |
| Joomun, A Saumtally, S Dookun-Saumtally, A. | |
| Detection and characterization of phytoplasmas from sugar cane and weeds | |
| 8th ISSCT Pathology Workshop, 23-27 January 2006, Petit-Bourg, Guadeloupe | |
| 8th ISSCT Pathology Workshop, Petit-Bourg, Guadeloupe, 23-27 January 2006, | |
| Conference paper | |
| 2006 | |
| p.21 | |
| Abstract only | |
| En | |
| En | |
| Sugarcane yellows is caused by sugarcane yellows phytoplasma (SCYP). It has been suggested that weeds growing in and around sugar cane fields may act as alternate hosts for the pathogen. In the present study, the polymerase chain reaction was used to assess the presence of the phytoplasma in both sugar cane and weeds in Mauritius. DNA was extracted from sugar cane and weed samples using the CTAB method. A nested-PCR approach was used to amplify a fragment of the 16S rDNA of the phytoplasma using universal primer pairs P1/P7 and R16F2n/R16R2. The expected 1250 bp fragment was amplified from sugar cane as well as weed samples including Cynodon dactylon, Sorghum verticilliflorum, and Eleusine indica. Restriction enzymes Rsa1 and Taq1 were used to determine the identity of the phytoplasmas present. The restriction profiles observed from phytoplasmas amplified from sugar cane varieties B 6504, B 50112 and SP716163 were different from SCYP groups previously described locally. For the phytoplasmas amplified from weeds, the restriction profile for Eleusine indica was similar to the new profile observed in sugar cane while the others were different suggesting diversity amongst phytoplasmas present both in sugar cane and weeds in Mauritius | |
| sugarcane diseases phytoplasmas characterization weeds | |
| Mauritius | |
| 2006-12-28 | |
| En | |
| Biotech | |
| Biotech path |