Genetic diversity in Ralstonia solanacearum strains from Mauritius using restriction fragment length polymorphisms

Code (CO)	MSI01P3535
Author (AU)	Dookun, A. Saumtally, S. Seal, S.
Title - English (ET)	Genetic diversity in Ralstonia solanacearum strains from Mauritius using restriction fragment length polymorphisms
Document Type(DT)	periodical article
Date of publication (DP)	2001
Series (SE)	J. Phytopath.
ISSN (SN)	0931-1785
Source (SO)	149(1): 51-55
Language of text (LT)	En
Language of summaries (LS)	En De
Abstract (AB)	Race 1, biovar III of Ralstonia (synonym Pseudomonas) solanacearum, causan organism of bacterial wilt, has been reported in Mauritius on several crops and plant species. The genetic relationship among 38 strains isolated from potato, tomato, bean and anthurium was determined by restriction fragment length polymorphisms (FRLPs). After hybridization with probe 5a67, five RFLP pattern could be distinguished. Types V and I were most commonly encountered. A common band of approximately 6.5kb was found in 35 strains. Type I pattern consisted of only this band and was observed in 12 out of 16 anthurium strains tested. Type V was associated with 12 out of 16 potato strains and consisted of a band of approximately 3.3kb in addition to the one observed in type I. FRLP patterns II, III, and IV were less frequently encountered. The RFLP analysis showed that genetic diversity was present in race 1, biovaar III strains. The relationship between the host and FRLP pattern is discussed.
Descriptors - English (DE)	Ralstonia solanacearum Pseudomonas solanacearum Bacterial wilt Genetic diversity Polymerase chain reaction Restriction fragment length polymorphisms
Descriptors - Geographic (DG)	Mauritius
Sort Key 1(K1)	Diseases
Sort Key 2 (K2)	Bacterial wilt
Date record entered (DA)	2001-02-13
Language of analysis (LA)	En
Location (LO)	Path
Processing status (PS)	CAT
MSIRI Staff (MS)	Path Biotech