Genotyping of sugar cane using molecular markers
| MSI06P4224 | |
| Parmessur, Y. Aljanabi, S. Narrainsawmy, V. Dookun-Saumtally, A. | |
| Genotyping of sugar cane using molecular markers | |
| Abstracts, 5th ISSCT Molecular Biology Workshop, 3-7 April 2006, Réduit, Mauritius | |
| Conference paper | |
| 2006 | |
| p.2.3 | |
| Abstract only | |
| En | |
| En | |
| The use of microsatellite marker systems in the genotyping of crop plants has now become a well-established practice across most molecular laboratories worldwide. This technology has been applied to study the genetic diversity of the noble canes, Saccharum officinarum. A collection of 125 noble canes, two wild varieties (S. spontaneum and S. robustum) and one clone of Erianthus sp. were screened with twenty radioactively labeled primers. A total of 215 polymorphic markers were scored, data was analyzed using the Numerical Taxonomy and Multivariate Analysis System (NTSYS-pc v 1.8) and a genetic distance dendogram was constructed using the Unweighted Pair Group Mean Arithmetic (UPGMA) clustering method. A high level of variability was observed among the noble canes with a coefficient of similarity ranging from 0.29 to 0.92. Clustering was also observed among varieties with similar geographical distribution. For example, the NG (New Guinea) varieties and the Tanna varieties were clustered into distinct groups with a high level of similarities across varieties. However, varieties from Mauritius (M) and Barbados (B) had a broader genetic base with a lower coefficient of similarity. Outgroups S. spontaneum, S. robustum and Erianthus sp. were considerably distinct from the noble canes. These results confirmed broad genetic base of sugar cane from which valuable resources can still be tapped and also provided a more targeted approach to the selection of parents for future breeding programs. | |
| sugarcane genotyping molecular markers | |
| Mauritius | |
| cane breeding and genetics | |
| 2006-12-28 | |
| En | |
| Biotech | |
| Biotech |