An improved and rapid protocol for the isolation of polysaccharide and polyphenol-free sugarcane DNA

Code (CO)	MSI00P3308
Author (AU)	Aljanabi, S. Forget, L. Dookun, A.
Title - English (ET)	An improved and rapid protocol for the isolation of polysaccharide and polyphenol-free sugarcane DNA
Document Type(DT)	Periodical article
Date of publication (DP)	1999
Series (SE)	Plant Mol. Biol. Rept.
Source (SO)	17: 1-8
Language of text (LT)	En
Language of summaries (LS)	En
Abstract (AB)	We have optimized a simple and rapid method for isolating milligram quantities of high quality DNA from polysaccharide- and polyphenolic-rich tissue such as sugarcane, lettuce and strawberry. The protocol utilizes fresh tissue culture without making use of liquid nitrogen or freese-drying for initial grinding of the tissue and it significantly minimize the use of lab materials. At least one hundred samples can be processed daily by one person. The isolated DNA is essentially free of polysaccharides, polyphenols, RNA and other contaminants, as judged by: its clear color, its viscosity, A260/A280 ratio, digestibility with restriction enzymes, and suitability for Restriction Fragment Length Polymorphism (RFLP)-and Polymerase Chain Reaction (PCR)-based techniques.
Descriptors - English (DE)	DNA Extraction Polyphenols polysaccharides RAPD Sugarcane Saccharum Methods Biotechnology
Descriptors - Geographic (DG)	Mauritius
Sort Key 1(K1)	Sugarcane
Sort Key 2 (K2)	Biotechnology
Date record entered (DA)	2000-01-07
Language of analysis (LA)	En
Affiliation (AF)	MSIRI
Location (LO)	Lib Biotech
Processing status (PS)	CAT
MSIRI Staff (MS)	Biotech