Race identification in the gumming disease pathogen Xanthomonas campestris pv vasculorum by fatty acid profiling
Code (CO)MSI99P1055
Author (AU)Dookun, A.
Stead, D.
Autrey, L. J. C.
Title - English (ET)Race identification in the gumming disease pathogen Xanthomonas campestris pv vasculorum by fatty acid profiling
Meeting (MT)3rd ISSCT Sugar Cane Pathology Workshop, Mauritius, 22-26 July 1991
Document Type(DT)Conference paper
Date of publication (DP)1991
Notes (NO)Abstract in Sug. Cane 1992 (1): 29
Language of text (LT)En
Language of summaries (LS)En
Abstract (AB)Fatty acid profiles were prepared from 83 isolates of Xanthomonas campestris pv vasculorum (XCV) obtained from sugar cane and other hosts in Mauritius and on a series of isolates obtained from the National Collection of Plant Pathogenic Bacteria (NCPPB). Isolates were grown on trypticase soy broth agar and after extraction, the fatty acids were analysed by gas-liquid chromatography and a special computer program. The XCV isolates were grouped on their principal component analysis. Race 1 isolates of Mauritius from sugar cane and palm formed a separate cluster from race 2 isolates. Moreover, isolates from the host Thysanolaena maxima gave a different profile as well as those isolates of the NCPPB ones originating from South Africa, Puerto Rico and Australia. It is inferred that fatty acid profiles can be used for race differentiation in DCV and to identify strains from other hosts and from various geographical regions. This technique can be of use in epidemiological studies within a given country or region.
Descriptors - English (DE)SUGARCANE
XANTHOMONAS CAMPESTRIS
GUMMOSIS
ANALYTICAL METHODS
BACTERIAL DISEASES
DISEASES
Descriptors - Geographic (DG)MAURITIUS
Sort Key 1(K1)Sugarcane: Diseases and disease management
Date record entered (DA)1992-07-15
Language of analysis (LA)En
Affiliation (AF)Mauritius Sugar Industry Research Institute, Reduit and Ministry of Agric., Food and Fisheries, UK.
MSIRI Staff (MS)PATH