Phosphate esters of the sugar-cane plant
Code (CO)MSI99P0025
Author (AU)Chan, P. Y.
Organisation (OR)University of London, London, GB
Title - English (ET)Phosphate esters of the sugar-cane plant
Thesis-Parent title (TH)Thesis, M.Sc.
Document Type(DT)Thesis
Date of publication (DP)1970
Source (SO)113 p.: 10 pl.,11 tbls., 5 figs.
Language of text (LT)En
Language of summaries (LS)En
Abstract (AB)A method is described for the extraction and purification of phosphate esters of the sugarcane. By the use of radioactive phosphate, an estimation of the relative amounts of these compounds was made possible. Good separation of these organic phosphates was obtained on paper-chromatography using the ascending technique in two dimensions at right angles. The pattern of phosphate esters in chromatographic resolution was similarly for both the roots and leaves but there were differences in the relative proportions of the individual esters. Significant amounts of some phosphate esters were labelled and identified even after a short period of 1 min. incubation of excised roots in the radioactive phosphate solution. In all the experiments, glucose-6-phosphate (G-6-P) was the dominant fraction of all the phosphate compounds. It was suggested that other esters became labelled at the expense of G-6-P with longer times of exposure in the radioactive phosphate solution. Phosphate uptake in the plant was stimulated at a certain level of aluminium but little effect was exerted by this element on the extent of phosphorylation in both the roots and leaves. With silicon, on the other hand, a marked increased in the degree of phosphorylation was noted although no effect was obtained on the phosphate uptake. Such an increase in the percentage of the organic phosphate fraction indicated that silicon pretreatment enhanced the metabolism of the plant; in fact, at least for sugarcane, it is found that silicon pretreatment gives better plant growth. Ammonium nitrogen was superior to nitrate nitrogen both in increasing the metabolic activities of the plant and in improving plant uptake of phosphate. Evidence was also obtained showing that treatment of detached roots with sucrose increased the proportion of the organic phosphate fraction. Fructose and glucose treated roots did not produce this effect.
Descriptors - English (DE)PHOSPHATES
ESTERS
SUGARCANE
PLANT NUTRITION
Descriptors - Geographic (DG)MAURITIUS
Sort Key 1(K1)Sugarcane: Soils and plant nutrition
Sort Key 2 (K2)Fertilization: Phosphorus
Call Number (CN)633.61:547.57
Date record entered (DA)1991-10-17
Language of analysis (LA)En
Affiliation (AF)Mauritius Sugar Industry Research Institute
Location (LO)LIB
Processing status (PS)CAT
Number of copies (NC)1
MSIRI Staff (MS)AGRIC ENG