Comparison of serological and inoculation methods for detecting latent infection of leaf scald
| MSI99P0436 | |
| Ricaud, C. Sullivan, S. Felix, S. Ferré, P. | |
| Comparison of serological and inoculation methods for detecting latent infection of leaf scald | |
| Periodical article | |
| 1978 | |
| Proc. int. Soc. Sug. Cane Technol. | |
| 16: 439-448 | |
| En | |
| En Es | |
| Serological and inoculation methods were compared for the diagnosis of leaf scald disease of sugarcane, particularly the detection of latent infection in cane stalks. An antiserum against Xanthomonas albilineans, the causal agent, was prepared by serial intravenous injections in a rabbit. Good serological reactions were obtained with pure culture whole-cell suspensions of the bacterium in tube agglutination, micro-agglutination and agar gel double diffusion tests. The antiserum had a high titre (1:2560) and was very specific. No reaction was obtained with five other bacterial plant pathogens including two infecting sugarcane: Xanthomonas vasculorum and Pseudomonas rubrisubalbicans. A slight reaction was obtained with another one not infecting sugarcane. For the diagnosis of the disease in cane stalks, these were sliced and allowed to exude in water for two hours. The exudates were strained through muslin and subjected to differential centrifugation: a high speed spin at 20,000 g, in between two low speed spins. The bacterium could be diagnosed in stalks showing disease symptoms by micro-agglutination tests with suspensions of the sediment, but the technique did not work for detection of the disease in stalks with latent infection. For the inoculation technique, the cane tissues were processed in the same way except that they were also homogenized and low speed centrifugation was omitted. The sediment was used for inoculating young shoots of a susceptible variety. The method was more sensitive than the serological technique and allowed the detection of latent infection. However further improvements to increase its reliability are desirable. | |
| SUGARCANE DIAGNOSTIC TECHNIQUES XANTHOMONAS ALBILINEANS XANTHOMONAS VASCULORUM DISEASES BACTERIAL DISEASES LEAF SCALD | |
| MAURITIUS | |
| Sugarcane: Diseases and disease management | |
| Bacterial diseases: Leaf scald | |
| 1992-02-23 | |
| En | |
| Mauritius Sugar Industry Research Institute | |
| LIB | |
| CAT | |
| PATH |