Improved serological methods for the diagnosis of the leaf scald bacterium, Xanthomonas albilineans
| MSI99P0113 | |
| Autrey, L. J. C. Dookun, A. Saumtally, S. | |
| Improved serological methods for the diagnosis of the leaf scald bacterium, Xanthomonas albilineans | |
| Periodical article | |
| 1990 | |
| Proc. int. Soc. Sug. Cane Technol. | |
| 20: 704-713 | |
| En | |
| En Fr Es | |
| The leaf scald bacterium was successfully diagnosed in crude and clarified sap and juice extracts by three serological methods with an antiserum having a titre of 1:2560. The tests allowed distinction of the organism from others causing gumming, mottled stripe and chlorotic streak which induce similar, when not identical, syndromes in sugar cane. The limits of detection of antigens from pure cultures were 1 x 10 5, 1 x 10 1, 1 x 10 0 cells per ml by immunofluorescence, latex flocculation and direct ELISA respectively. Latent infection was detected only by the direct ELISA method. The advantages and disadvantages of the methods used are discussed. The importance of using simple but sensitive as well as advanced methods for the disease diagnosis in sugar cane is emphasized. | |
| SEROLOGICAL DIAGNOSIS XANTHOMONAS ALBILINEANS DIAGNOSTIC TECHNIQUES ENZYME LINKED IMMUNOSORBENT ASSAY IMMUNOFLUORESCENCE FLOCCULATION BACTERIAL DISEASES DISEASES ANALYTICAL METHODS | |
| MAURITIUS | |
| Sugarcane: Diseases and disease management | |
| Bacterial diseases: Leaf scald | |
| 1992-02-07 | |
| En | |
| Mauritius Sugar Industry Research Institute | |
| LIB | |
| CAT | |
| PATH |