Race differentiation in Xanthomonas campestris pv vasculorum by restriction fragment length polymorphisms
| MSI99P0161 | |
| Saumtally, S. Autrey, L. J. C. | |
| Race differentiation in Xanthomonas campestris pv vasculorum by restriction fragment length polymorphisms | |
| Periodical article | |
| 1990 | |
| Sug. Cane | |
| (1): 13-17 | |
| En | |
| En Fr Es | |
| DNA restriction fragment length polymorphisms (RFLPs) were evaluated as a tool for distinguishing among three races of Xanthomonas campestris pv vasculorum (Xcv), causal agent of gumming disease of sugar cane. Two probes, pUFT-1 and pUFT-11, derived from X.c. pv citri, and six restriction enzymes, EcoR I, Hind III, Bgl II, Xho I, Bam H I and Ava I, were used. RFLPs were detected among the three races with bands specific to race 2, after digestion with enzyme Ava I and hybridization with probe pUFT-1. RFLPs were not found with the other enzyme-probe combinations. Races 1 and 3 could not be distinguished. The results indicated that the three races were genetically closely related and that, to detect small variations in their chromosomal DNA, probes derived from a genomic DNA library of Xcv will have probably to be used. | |
| SUGARCANE XANTHOMONAS CAMPESTRIS DNA GUMMOSIS DISEASES BACTERIAL DISEASES | |
| MAURITIUS | |
| Sugarcane: Diseases and disease management | |
| Bacterial diseases: Gumming | |
| 1992-02-11 | |
| En | |
| Mauritius Sugar Industry Research Institute | |
| LIB | |
| CAT | |
| 1 | |
| PATH |