The HPLC analysis of flower pigments in zonal Pelargoniums
| MSI99P0045 | |
| Bissessur, D. | |
| University of Wales, GB | |
| The HPLC analysis of flower pigments in zonal Pelargoniums | |
| Thesis, MSc | |
| Thesis | |
| 1990 | |
| 79 p. | |
| En | |
| A brief review is given of flower pigments. Colour matching (using RHS colour chart), extraction and separation of flower pigments was performed on seven cultivars of zonal pelargoniums. Colour matching was not easy especially when two flowers were alike; however, every effort was made to achieve consistency. Each cultivar had a specific flower colour, and their extracts also differed. Pigments were extracted by grinding fresh flower petals in liquid nitrogen and suspending the resulting powder acidified methanol (0.5 per cent (v/v) concentrated HCl). After homogenisation followed by filtration, the extract was concentrated using a rotary evaporator at 30°C. The dried extract was dissolved in 23 per cent of the solvent used for HPLC analysis. Each sample was passed through Waters 0.45 micrometre millipore filter prior to HPLC analysis. The pigments were separated by gradient elution using a modified modus operandi of Asen and Griesbach (1983). The two authentic markers used were malvidin and pelargonidin. Two or more peaks were observed for each cultivar indicating the presence of several pigments, which varied in quantity as shown by the different peak areas and retention times. The problems faced are discussed and solutions proposed. | |
| CHROMATOGRAPHY PELARGONIUM PIGMENTS FLOWERS HPLC | |
| Pelargonium | |
| Physiology | |
| 1991-10-28 | |
| En | |
| Mauritius Sugar Industry Research Institute | |
| PB |