Identification and characterization of pathogens associated with yellow leaf syndrome of sugar cane
Code (CO)MSI03P3908
Author (AU)Parmessur, Y.
Aljanabi, S.
Dookun-Saumtally, A.
Title - English (ET)Identification and characterization of pathogens associated with yellow leaf syndrome of sugar cane
Thesis-Parent title (TH)Lalouette, J. A., Bachraz, D. Y., Bheenick, K. J.(eds), Proceedings Sixth Meeting of Agricultural Scientists, Boname Hall, MSIRI, Réduit, Mauritius, 8-9 May 2003. Réduit: Food and Agricultural Research Council. 340 p.
Document Type(DT)book chapter
Date of publication (DP)2004
Source (SO)p. 1-6
Notes (NO)Also appeared in Revue agric. sucr. Ile Maurice 84(1-3): 89-94 (2005)
Language of text (LT)En
Language of summaries (LS)En
Abstract (AB)Yellow leaf syndrome (YLS) of sugar cane is characterized by the yellowing of the midrib followed by the leaf blades in mature canes. Two pathogens have been associated with the disease; a luteovirus, Sugar cane yellow leaf virus (SCYLV) and a phytoplasma, sugar cane yellows phytoplasma (SCYP). Reverse transcriptase polymerase chain reaction (RT-PCR) was used to identify the virus while a nested-PCR was found to be reliable for the detection of the phytoplasma. The two pathogens were observed in sugar cane plants with or without symptoms and they also co-existed in a number of varieties. A strong correlation was found between the presence of the phytoplasma and YLS symptoms when compared to the presence of the virus. Restriction fragment length polymorphism (RFLP) analysis of the phytoplasma 16S rRNA amplified product demonstrated the presence of at least three phytoplasma groups in sugar cane in Mauritius.
Descriptors - English (DE)sugarcane
diseases
yellow leaf syndrome
luteovirus
Virus diseases
phytoplasma
RT-PCR
nested-PCR
RFLP
Sugarcane yellow leaf virus
phytoplasma
sugarcane yellows phytoplasma
Restriction fragment length polymorphism
Reverse transcriptase polymerase chain reaction
diagnostic techniques
Descriptors - Geographic (DG)Mauritius
Sort Key 1(K1)Sugarcane: Diseases and disease management
Sort Key 2 (K2)Virus diseases: Yellow leaf syndrome
Date record entered (DA)2003-05-09
Language of analysis (LA)En
Affiliation (AF)MSIRI
Location (LO)LIB
Processing status (PS)cat
MSIRI Staff (MS)BIOTECH