Comparative mapping of sugarcane using sorghum resources
| MSI11P4584 | |
| Parmessur, Y Punna, R Hash, T Dookun-Saumtally, A Puchooa, D | |
| Comparative mapping of sugarcane using sorghum resources | |
| Book of Abstracts: University of Mauritius Research Week 2011, University of Mauritius, Réduit, 5-9 September 2011 | |
| University of Mauritius Research Week 2011, University of Mauritius, Réduit, 5-9 September 2011 | |
| Book chapter | |
| 2011 | |
| p. 15 (Agriculture) | |
| Réduit, University of Mauritius | |
| Abstract only | |
| En | |
| En | |
| QTL mapping in sugarcane (Saccharum spp) is a major challenge owing to its large genome size (10Gb) and its genetic complexity. A partial linkage map of sugarcane variety M 134/75 was previously constructed containing 557 AFLP and genomic SSR markers. A major QTL for sugarcane yellow spot (YS) disease resistance, explaining 24 per cent of observed phenotypic variation for the disease resistance, was identified. Comparative mapping beween sorghum and sugarcane is further being exploited to fine-map this QTL with the aim of introducing marker assisted selection for YS resistance in the sugarcane breeding programme. A set of 425 sorghum EST-SSR primer pairs was tested for its suitability to act as anchor markers beween the sugarcane and sorghum genomes. These were screened on the mapping parents M 134/75 and R 570. The genetic similarity between the sorghum and sugarcane genomes was confirmed by 95 per cent marker transferability across the two species, with 295 sorghum EST-SSR primers detecting polymorphism for the mapping parent M 134/75 and with an average of 2.6 polymorphisms per primer pair. The progeny population of 226 individuals was genotyped with 37 sorghum EST-SSR primers from which 102 single-dose coding markers were scored. These were combined with 985 available non-coding markers and analyzed by GMendel to produce an enhanced map of M 134/75 containing 143 linkage groups (LGs). In this map were integrated 58 sorghum EST-SSR markers, distributed across 26 LGs. Evidence of gene and chromosome duplication events contributing to the expansion of the Saccharum was observed. COnservation of gene order in sugarcane, sorghum and rice (in silico) was also exemplified in a number of LGs. LG 68 containing the YS resistance QTL was enhanced with three sorghum markers, Xisep1225, Xisep0203 and Xisep0612. Using the Phytozome and the Gbrowse software (http://www.phytozome.net/sorghum.php), LG 68 was found syntenic to sorghum chromosome SBI-10. Effort is being focused on the transfer of SBI-10-linked EST-SSR markers to LG 68 in order to identify markers tightly linked to the YS resistance QTL. | |
| sugarcane sorghum comparative map Saccharum spp. QTL mapping EST-SSR variety M 134/75 genome mapping molecular markers | |
| Mauritius | |
| Cane breeding and genetics | |
| Genetic techniques and protocols | |
| 2011-09-14 | |
| En | |
| LIB | |
| CAT | |
| BIOTECH |