Application of biotechnology to sugarcane pathogens
| MSI99P3086 | |
| Autrey, L. J. C. Saumtally, S. Dookun, A. | |
| Application of biotechnology to sugarcane pathogens | |
| Periodical article | |
| 1998 | |
| Indian Sugar | |
| 47 (10): 789-798 | |
| (Also published in Proceedings ISSCT 22: 15-22, 1996 | |
| En | |
| En | |
| Biotechnology has resulted in several developments in the field of plant pathology since the early 1980s. Techniques based on monoclonal antibodies and molecular biology such as DNA-DNA hybridization, DNA sequencing, restriction fragment length polymorphism (RFLP) analysis, and polymerase chain reaction (PCR) have been applied in this field. The main uses of these tools include a more precise and sensitive diagnosis of diseases, safer germplasm movement, production of clean seed, identification of new strains, establishment of new taxonomic relationships and monitoring of epidemics. RFLP analysis has proved useful for diseases such as gumming, for which races have been differentiated and strains assigned to various geographic regions. For the leaf scald bacterium, polymorphisms have been observed among strains within and across serotypic groups. PCR has been applied to several viruses- namely, mosaic, bacilliform, streak and Fiji - and lately to fungi such as smut and Pythium spp. This technique is also developed for luteoviruses are being used for detection. A simplified version of PCR consisting of the use of random primers (random amplified polymorphic DNA-RAPD) has been developed for ratoon stunting disease, Pachymetra root rot and white leaf. Progress has also been achieved in generating monoclonal antibodies against gumming and leaf scald bacteria as well as streak and mosaic viruses. The application of biotechnology tools to sugarcane pathogens is steadily increasing but is still at an early stage of development for routine use. However, the inherent potential advantages of these techniques are overwhelming and their widespread adoption looks promising. | |
| SUGARCANE BIOTECHNOLOGY PATHOGENS MOLECULAR BIOLOGY DIAGNOSIS RESTRICTION FRAGMENT LENGTH POLYMORPHISM POLYMERASE CHAIN REACTION RANDOM AMPLIFIED POLYMORPHIC DNA | |
| MAURITIUS | |
| Biotechnology | |
| 1998-09-07 | |
| En | |
| LIB | |
| CAT | |
| PATH BIOTECH |