Application of microsatellite markers to the sugarcane breeding programme

Code (CO)	MSI05P4158
Author (AU)	Joomun, N Parmessur, Y Dookun-Saumtally, A
Title - English (ET)	Application of microsatellite markers to the sugarcane breeding programme
Thesis-Parent title (TH)	Lalouette, J. A., Bheenick, K. J. and Nundalallee, C. (eds), Proceedings, Seventh Meeting of Agricultural Scientists, 4-6 May 2005, Boname Hall, MSIRI, Mauritius. (Draft)
Document Type(DT)	book chapter
Date of publication (DP)	2005
Source (SO)	p. 77-86
Publisher (PB)	Réduit: Food and Agricultural Research Council
Notes (NO)	In: Book of Abstracts. Seventh Meeting of Agricultural Scientists, 4-6 May 2005, Boname Hall, MSIRI, Réduit, Mauritius. p 22 (Proceedings available at http://www.gov.mu/portal/sites/ncb/moa/farc/amas2005/pdf/MAS20051stDraft.pdf)
Language of text (LT)	En
Language of summaries (LS)	En
Abstract (AB)	Microsatellite markers or simple sequence repeats (SSRs) consist of short and repeated DNA sequences (1-6 nucleotides) densely interspersed throughout the eukaryotic genomes. They are regarded as the marker of choice for a number of plant species because they are highly polymorphic and species specific. Here we report the application of microsatellite markers in the sugarcane breeding programme. Six SSR primer pairs were selected based on their high level of polymorphisms. A non-radioactive fingerprinting method using fluorescently labelled SSR primers on an ABI 310 Genetic Analyzer was optimised. The primers proved useful for the identification of cultivars and in the verification of true to type clones regenerated from callus culture. The identification of male parents in polycrosses was also possible using these primer pairs. The hybrid nature of clones derived from intergeneric crosses (Saccharum officinarum x Erianthus arundinaceus) was also investigated using microsatellite markers and further confirmed by PCR using specific primers to the 5s ribosomal DNA spacer region.
Descriptors - English (DE)	sugarcane breeding genetic improvement genetics Microsatellites SSR fingerprinting intergeneric hybrids Saccharum Erianthus 5s rRNA microsatellite markers
Descriptors - Geographic (DG)	Mauritius
Sort Key 1(K1)	Cane breeding and genetic improvement
Sort Key 2 (K2)	Genetic techniques and protocols
Date record entered (DA)	2005-05-03
Language of analysis (LA)	En
Processing status (PS)	LIST
MSIRI Staff (MS)	Biotech