An alternative approach for micropropagating of sugar cane using leaf discs as explants
| MSI06P4223 | |
| Mulleegadoo, K. Dookun-Saumtally, A. | |
| An alternative approach for micropropagating of sugar cane using leaf discs as explants | |
| Abstracts, 5th ISSCT Molecular Biology Workshop, 3-7 April 2006, Réduit, Mauritius | |
| Conference paper | |
| 2006 | |
| p.5.1 | |
| Abstract only | |
| En | |
| En | |
| Micropropagation of sugar cane has mostly been effected using buds or shoot tips as explants. Use of these explants, however, can result in severe oxidation or contamination with saprophytic microorganisms especially when initiated from mother plants that have been in the field for a number of years. An alternative method for the production of in vitro plantlets of sugar cane from such mother plants, using leaf discs as explants, was therefore investigated. Young leaf discs from three commercial sugar cane varieties, M 3035/66, S 17 and R 570, were dissected from surface-sterilized tops and cultured on callus-induction medium containing 3 mg/L 2,4-D for a period of two weeks. They were then cultured on regeneration medium containing 2 mg/L Kinetin and 5 mg/L NAA for four to six weeks before being transferred onto hormone-free solid medium. When shoots were about two to three cm high, they were transferred to liquid medium. Subculture onto fresh medium was effected every two to three weeks and shoot development was monitored over a period of 22 to 24 weeks. Six cane tops were used for each variety and twenty leaf discs per cane top were cultured. Prolific shoot regeneration occurred in all three varieties. Contamination and oxidation were minimal and more than 70 per cent of the leaf discs cultured formed shoots in all varieties. About 400 and 280 shoot clumps were formed in varieties M 3035/66 and S 17 respectively whilst for variety R 570, some 250 clumps were regenerated. This study, therefore, demonstrates the potential of using leaf disc culture for micropropagation of sugar cane, especially for recalcitrant varieties that cannot be initiated from buds or shoot tips as a result of oxidation or contamination. | |
| sugarcane micropropagation | |
| Mauritius | |
| cane breeding and genetics | |
| 2006-12-28 | |
| En | |
| Biotech | |
| Biotech |