Sugar cane bacilliform virus: symptomatology, diagnosis and distribution in cane germplasm in Mauritius
| MSI99P0104 | |
| Autrey, L. J. C. Boolell, S. Lockhart, B. E. L. | |
| Sugar cane bacilliform virus: symptomatology, diagnosis and distribution in cane germplasm in Mauritius | |
| 3rd ISSCT Sugar Cane Pathology Workshop, MSIRI, Reduit, 22-26 July 1991 | |
| Conference paper | |
| 1991 | |
| Abstract in Sug. Cane 1991 (6): 9. | |
| En | |
| En | |
| Sugar cane bacilliform virus (SCBV), first described as a latent virus in variety Mex 57473 in Morocco, was found in Mauritius to be consistently associated with leaf freckling in 127 out of 128 noble canes and in a large number of interspecific hybrids. Noble canes were grouped into five categories on the basis of symptoms. Freckling was of varying intensity and in noble canes ranged from mild to severe chlorosis with a mosaic pattern particularly apparent in varieties Iscambine Striped and Bambou Striped. In a collection of interspecific hybrids of diverse geographical origin, leaf freckles were absent in 13 clones and were of mild, slight and moderate intensity in 362, 54 and 8 clones, respectively. SCBV was diagnosed in 104 noble cane having leaf freckles and in the commercial and promising varieties S17, M791/75 and M13/56 by DAS ELISA, using an antiserum to banana streak virus (BSV). It could not be diagnosed with an antiserum to a sugar cane isolate from Mex 57473 from Morocco. By the same method, the highest concentration of virus particles was found in the youngest leaf in variety Iscambine Striped. In 21 out of 24 noble canes tested, virus particles were more abundant in the first rather than the fourth leaf. The middle of the leaf contained generally more virus particles than its base or tip. Hence, for routine diagnosis of SCBV by DAS ELISA, the middle part of the youngest leaf is used. Attempts at diagnosing SCBV by indirect ELISA were unsuccessful. Direct examination of crude sap in the electron microscope by negative staining was unsatisfactory for detection of SCBV except when used with two noble canes. However, by immune electron microscopy (IEM), virus particles were readily detected with antisera to the sugar cane isolate from Mex 57473 and BSV. A relationship was found by IEM between severity of symptoms and concentration of virus particles in noble cane but not in interspecific hybrids. Some noble canes and interspecific hybrids with varying intensity of freckles, though negative by ELISA, were found to contain SCBV particles by IEM in 11 clones from Argentina, Barbados, Brazil, Puerto Rico, Reunion and USA. It is inferred that SCBV is not, except in certain clones, a latent virus originally thought and that the most reliable te chnique for its detection is IEM. The virus, which appears to be widely distributed in sugar cane germplasm around the world, exists probably as host-adapted strains, a matter which is under investigation. | |
| SUGARCANE PLANT VIRUSES SYMPTOMATOLOGY DIAGNOSIS DISTRIBUTION DISEASES SCBV SUGAR CANE BACILLIFORM VIRUS | |
| MAURITIUS | |
| Sugarcane: Diseases and disease management | |
| Viruses: SCBV | |
| 1992-01-21 | |
| En | |
| Mauritius Sugar Industry Research Institute University of Minnesota. Department of Plant Pathology, St Paul, USA | |
| LIB | |
| CAT | |
| PATH |