Sugarcane leaf yellows phytoplasma in Mauritius: Molecular characterisation, transmission and alternative hosts
| MSI07P4276 | |
| Joomun, N Dookun-Saumtally, A Saumtally, S Ganeshan, S | |
| Sugarcane leaf yellows phytoplasma in Mauritius: Molecular characterisation, transmission and alternative hosts | |
| periodical | |
| 2007 | |
| Proc. int. Soc. Sug. Cane Technol. | |
| 26: 1005-1013 | |
| En | |
| En Sp Fr | |
| Sugarcane leaf yellows phytoplasma (SCYP), which causes leaf yellows (LY) disease, is commonly encountered in Mauritius. The objectives of this study were (1) to identify the vector transmitting the phytoplasma (2) to characterize the phytoplasma and (3) to identify alternate hosts harbouring the phytoplasma in sugar cane fields. Screening of nucleic extracts from the sugar cane Delphacid, Perkinsiella saccharicida by nested polymerase chain reaction (PCR) using universal primers revealed the presence of phytoplasmas. Molecular characterization using restriction enzymes showed that infection in the planthopper could be due to the presence of more than one phytoplasma. Two PCR products amplified from P. saccharicida were sequenced and found to belong to two separate groups namely the 16SrIII-Western X group and the aster yellows 16SrI group of phytoplasmas. The former group was also observed in sugar cane and was not previously recorded in this crop in Mauritius. These results indicate the possible involvement of P. saccharicida as a vector of SCYP. The PCR technique was also used to diagnose phytoplasmas in graminaceous weeds growing in sugar cane fields. Fragments related to the Western X group were amplified from Sorghum verticilliflorum. | |
| sugarcane phytoplasmas sugarcane leaf yellows Yellow leaf syndrome diseases characterisation vectors disease transmission Perkinsiella saccharicida Plant hoppers | |
| Mauritius | |
| Sugarcane: Diseases and disease management | |
| Phytoplasma: Sugarcane yellows phytoplasma | |
| 2007-08-17 | |
| En | |
| LIB | |
| CAT | |
| BIOTECH Path Ento |