Evaluation of the genetic base of sugarcane cultivars and structuration of the diversity at the chromosome level using molecular markers
Code (CO)MSI99P3244
Author (AU)Jannoo, N.
Grivet, L.
Dookun, A.
d'Hont, A.
Glaszmann, J. C.
Title - English (ET)Evaluation of the genetic base of sugarcane cultivars and structuration of the diversity at the chromosome level using molecular markers
Thesis-Parent title (TH)Lalouette, J. A., Bachraz, D. Y., and Sukurdeep, N. (eds), Proceedings of the fourth Annual Meeting of Agricultural Scientists, Boname Hall, MSIRI, Réduit, Mauritius, 21-22 October 1999.
Document Type(DT)book chapter
Date of publication (DP)2001
Source (SO)p. 123-135
Publisher (PB)Réduit: Food and Agricultural Research Council (FARC)
Language of text (LT)En
Abstract (AB)Molecular diversity was analysed among 160 sugarcane clones consisting of 109 modern cultivars mainly from Barbados and Mauritius and 51 of S. officinarum species, which is the major source of genes in modern cultivars. Twelve low copy nuclear rflp probes scattered over the genome were applied. Although only a few clones of S. officinarum were used in the genealogy of sugarcane cultivars, they encompassed most of the diversity observed within this species. Moreover, more than 80 per cent of the markers present in the whole S. officinarum sample were also prsent in cultivars due probably to high heterozygosity and polyploidy. A distinct separation was observed between the two main groups of cultivars, Barbados and Mauritius. This appeared essentially due to S. spontaneum alleles present in mauritian cultivars and absent in Barbadan ones. Linkage disequilibrium was also investigated in a population of 59 cultivars using 38 RFLP probes regularly distributed over the sugarcane genome map. Associations were observed between 33 loci separated by less than 10 cM. This disequilibrium is interpreted as the result of the foundation bottleneck related to the history of sugarcane cultivars. A practical application is that markers can be used to track known QTL in modern breeding materials without the necessity to repeatedly study segretating progenies.
Descriptors - English (DE)SUGARCANE
GENETIC ENGINEERING
MOLECULAR MARKERS
DIVERSITY
POLYPLOIDY
RFLP
LINKAGE DISEQUILIBRIUM
Descriptors - Geographic (DG)Mauritius
Sort Key 1(K1)Cane breeding and genetics
Sort Key 2 (K2)Biotechnology
Date record entered (DA)1999-10-14
Language of analysis (LA)En
Affiliation (AF)MSIRI
Processing status (PS)cat
MSIRI Staff (MS)Biotech