Detection and characterisation of sugarcane yellows phytoplasma
| MSI01P3571 | |
| Aljanabi, S. M. Parmessur, Y. Jones, P. Saumtally, S. Dookun, A. | |
| Detection and characterisation of sugarcane yellows phytoplasma | |
| 24th ISSCT Congress, Brisbane, September 01 | |
| periodical article | |
| 2001 | |
| Proc. int. Soc. Sug. Cane Technol. | |
| 24(2): 343-348 | |
| En | |
| En Fr Es | |
| A study was carried out to assess the distribution and association of sugarcane yellows phytoplasma-ScYP in sugarcane affected by yellow leaf syndrome (YLS) in Mauritius. The polymerase chain reaction was used to detect and characterize the phytoplasma. Samples were collected from clones undergoing quarantine, in a variety collection plot, in mature commercial fields and from 6 months old commercial fields. A 1.25 kb DNA fragment encoding for the phytoplasma 16S rRNA was consistently amplified by nested-PCR. Of the 204 samples with and without symptoms derived from 166 varieties, 95 (57 per cent) tested positive for ScYP by PCR. Restriction fragment length polymorphism analysis of the phytoplasma 16S rDNA amplified product indicated that 3 phytoplasma groups are present in sugarcane in Mauritius. The results indicated that detection of YLS based on symptoms only is not reliable since large number of asymptomatic varieties were positive for the phytoplasma early in the growing season. | |
| sugarcane yellows phytoplasma PCR Polymerase chain reaction quarantine yellow leaf syndrome Phytoplasma | |
| Mauritius | |
| Sugarcane: Diseases and disease management | |
| Sugarcane Yellows Phytoplasma | |
| 2001-09-10 | |
| En | |
| Biotech Path |