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Identification of a major quantitative trait locus (QTL) for yellow spot (Mycovellosiella koepkei) disease resistance in sugarcane
Code (CO)MSI06P4220
Author (AU)Aljanabi, S. M.
Parmessur, Y.
Kross, H.
Dhayan, S.
Saumtally, S.
Ramdoyal, K.
Autrey, L. J. C.
Dookun-Saumtally, A.
Title - English (ET)Identification of a major quantitative trait locus (QTL) for yellow spot (Mycovellosiella koepkei) disease resistance in sugarcane
Document Type(DT)periodical article
Date of publication (DP)2007
Series (SE)Molecular Breeding
Source (SO)19(1): 1-14
Notes (NO)Published online at: http://dx.doi.org/10.1007/s11032-006-9008-3
Language of text (LT)En
Language of summaries (LS)En
Abstract (AB)In this study we used amplified fragment length polymorphism (AFLP) and microsatellite (short sequence repeat or SSR) markers to identify a major quantitative trail locus (QTL) for yellow spot (Mycovellosiella koepkei) disease resistance in sugarcane. A bi-parental cross between a resistant variety, M 134/75, and a susceptible parent, R 570, generated a segregating population of 227 individuals. These clones were evaluated for yellow spot infection in replicated field trials in two locations across two consecutive years. A x2-test (x2 at 98 per cent confidence level) of the observed segregation pattern for yellow spot infection indicated a putative monogenic dominant inheritance for the trait with a 3 (resistant):1(susceptible) ratio. The AFLP and SSR markers identified 666 polymorphisms as being present in the resistant parent and absent in the susceptible one. A genetic map of M 134/75 was constructed using 557 single-dose polymorphisms, resulting in 95 linkage groups containing at least two markers based on linkages in coupling. QTL analysis using QTLCartographer v1.17d and MAPMAKER/QTL v1.1 identified a single major QTL located on LG87, flanked by an AFLP marker, actctc10, and an SSR marker, CIR12284. This major QTL, which was found to be linked at 14 cM to an AFLP marker, was detected with LOD 8.7, had an additive effect of -10.05 per cent and explained 23.8 per cent of the phenotypic variation of yellow spot resistance.
Descriptors - English (DE)Linkage mapping
Molecular markers
Mycovellosiella koepkei
Polyploidy
Quantitative resistance
Sugarcane
diseases
yellow spot disease
Descriptors - Geographic (DG)Mauritius
Sort Key 1(K1)Sugarcane: Diseases and disease management
Sort Key 2 (K2)Fungal diseases: Yellow spot
Date record entered (DA)2006-12-28
Language of analysis (LA)En
Location (LO)BIOTECH
Lib
MSIRI Staff (MS)Biotech
Path
PB