Genetic polymorphism in Xanthomonas albilineans strains originating from 11 geographical locations, revealed by two DNA probs
Code (CO)MSI00P3507
Author (AU)Jaufeerally-Fakim, Y.
Autrey, J. C.
Daniels, M. J.
Dookun, A.
Title - English (ET)Genetic polymorphism in Xanthomonas albilineans strains originating from 11 geographical locations, revealed by two DNA probs
Document Type(DT)Periodical article
Date of publication (DP)2000
Series (SE)Letters Appl. Microbiol.
Source (SO)30: 281-283
Language of text (LT)En
Language of summaries (LS)En
Abstract (AB)Two DNA fragments from Xanthomonas albilineans were used as probes to study the molecular diversity among strains of this pathogen. Two serologically distinct groups, serovars I and II, could be differentiated by hybridization to the probes. these probes, designated 830 and 838, were cloned after subtractive DNA hybridization of common sequences of xanthomonas campestris pv. vasculorum from a serovar I strain of X. albilineans. They did not hybridize to the DNA of several other xanthomonads or to sugarcane DNA under the conditions of hybridization used. faint bands were observed upon hybridization of probe 830 with one strain of X. campestris pv. phaseoli. The same banding patterns were obtained with a strain of X. albilineans from burkina Faso and the serovar II strains of Mauritius. The serovar I strains from Mauritius and two other strains each from Reunion and South Africa had similar pattern.
Descriptors - English (DE)SUGARCANE
XANTHOMONAS albilineans
Leaf Scald
ANALYTICAL METHODS
BACTERIAL DISEASES
DISEASES
DNA probes
Subtractive hybridization
Genomic subtraction
Genetic polymorphism
Descriptors - Geographic (DG)MAURITIUS
Sort Key 1(K1)Sugarcane
Sort Key 2 (K2)Diseases - Bacterial diseases
Date record entered (DA)2000-12-05
Language of analysis (LA)En
Affiliation (AF)Mauritius Sugar Industry Research Institute, Reduit and Ministry of Agric., Food and Fisheries, UK.
Location (LO)Biotech
Processing status (PS)CAt
MSIRI Staff (MS)Biotech