Identification of molecular markers associated with yellow spot resistance in sugarcane

Code (CO)	MSI00P3420
Author (AU)	Aljanabi, S. M. Dookun, A. Saumtally, S. Domaingue, R.
Title - English (ET)	Identification of molecular markers associated with yellow spot resistance in sugarcane
Document Type(DT)	Periodical article
Date of publication (DP)	2000
Series (SE)	Sugar Cane International
Source (SO)	(Jun 2000): 6-11
Language of text (LT)	En
Language of summaries (LS)	En
Abstract (AB)	Yellow spot of sugarcane is the most important leaf disease in the super humid zone of Mauritius. A study was carried out from 1994-98 to identify molecular markers linked to the disease resistance. Infection rate was recorded during the peak infection of the year in plant cane and two ratoons in the mapping population consisting of 80 progeny derived from a resistant variety M 596/78 and susceptible clone M 937/77. Random amplified polymorphic DNA was used to identify single-dose polymorphisms, which were used to build a genetic linkage map of a resistant variety M 596/78. One hundred and forty 10-mer oligonucleotides primers of arbitrary sequence were selected from a pool of 280 primers screened on the parents. Two hundred and fifty six polymorphisms were detected in the mapping population and 134 were single dose polymorphisms (i.e they displayed 1:1 segregation, c2 at 98 per cent confidence level). Linkage analysis (LOD = 5.0 at 30 cM cut off distance for two point analysis and LOD = 4.0 at 37 cM for three and multipoint analysis) for single-dose markers placed them into 20 linkage groups containing at least 2 markers. These markers spanned over 1065 contiguous centimorgans (cM) with 68 markers remaining unlinked. Association of 134 single-dose markers with quantitative trait loci (QTL) of disease infection rate was determined using non-parametric analysis and QTL Cartographer software. QTL analysis revealed two markers significantly (P less than 0.05) associated with disease resistance and they explained 20 per cent of the total phenotypic variation. In addition, two other markers were significantly (P less than 0.01 and 0.001 respectively) associated with susceptibility to yellow spot and they explained 27 per cent of the total phenotypic variation. This is the first molecular study of the association of QTL to yellow spot resistance.
Descriptors - English (DE)	Sugarcane Yellow spot RAPD Molecular markers Genetic map Mycovellosiella koepkei
Descriptors - Geographic (DG)	Mauritius
Sort Key 1(K1)	Sugarcane: Diseases and disease management
Sort Key 2 (K2)	Yellow spot
Date record entered (DA)	2000-08-31
Language of analysis (LA)	En
Location (LO)	Lib
Processing status (PS)	Cat
MSIRI Staff (MS)	Biotech Path PB