Evaluation of genetic diversity in Saccharum officinarum clones using microsatellite markers

Code (CO)	MSI04P3962
Author (AU)	Aljanabi, S. Parmessur, Y. Dookun-Saumtally, A.
Title - English (ET)	Evaluation of genetic diversity in Saccharum officinarum clones using microsatellite markers
Meeting (MT)	4th ISSCT Molecular Biology Workshop, CIRAD, Montpellier, France, 7-11 April 2003
Document Type(DT)	Conference paper
Date of publication (DP)	2003
Abstract (AB)	Microsatellites or simple sequence repeats (SSRs) are highly plymorphic and efficient markers for evaluating genetic diversity and determining cultivar identity. SSRs have recently been applied to sugarcane and their potential was investigated in this study to determine the genetic diversity as well as the identity of clones within a diverse collection of noble canes (Saccharum officinarum) available in Mauritius. In this collection it was suspected that a number of clones differently labelled could be of the same origin. One hundred and fifteen cultivars of interest to Mauritius Sugar Industry Research Institute (MSIRI) breeding programme were evaluated by means of 10 microsatellite primers. One PCR primer was radiolabelled with 33P dATP and amplified alleles were resolved on 5 per cent polyacrylamide-denaturing gel. A total of 143 polymorphic markers were scored and the number of alleles per marker ranged from 7-21, with an average of 14.3. Polymorphisms were detected at all loci with a diversity value ranging from 0.68-0.96 and an average of 0.91. A significant positive correlation (P<0.01) was found between the number of repeats and the number of alleles as well as between the number of alleles and the diversity index. The results indicated that the collection of noble canes is genetically highly structured with a range of 0.29-0.92 genetic similarity among the clones. Fingerprinting analysis indicated that the noble canes fell into several groups. Distinct groups could be observed such as Tanna, Bamboo, NG (New Guinea) and B (Barbados) while cultivars with M (Mauritius) designation showed much variation amongst them. Two clones labelled RP6 and RP6S were found genetically identical. The results also suggested that a relatively small number of microsatellite markers could be used for the estimation of genetic diversity and the identification of Saccharum officinarum clones.
Descriptors - English (DE)	Sugarcane Saccharum officinarum genetic diversity biotechnology molecular markers
Sort Key 1(K1)	Sugarcane
Sort Key 2 (K2)	Biotechnology
Date record entered (DA)	2004-01-28
Processing status (PS)	LST
MSIRI Staff (MS)	BIOTECH