Genetic transformation of two Mauritian sugarcane varieties for resistance tot he herbicide Basta
| MSI09P4339 | |
| Mulleegadoo, K. Dookun-Saumtally, A. | |
| Genetic transformation of two Mauritian sugarcane varieties for resistance tot he herbicide Basta | |
| periodical article | |
| 2009 | |
| Sug. Cane Int. | |
| 27(2): 65-69 | |
| En | |
| En | |
| Two transgenic Mauritian sugarcane varieties, M 292/70 and M 3035/66, resistant to the herbicide Basta (glufosinate-ammonium) were obtained by microprojectile bombardment of embryogenic callus using plasmid pAHC25. This construct contains the bar and [beta]-glucuronidase (gus) genes both under the control of the maize ubiquitin (Ubi) promoter. Targets were placed at a distance of 6 cm from the macrocarrier launch assembly and bombarded at a pressure of 1550 psi. They were osmotically treated prior to and after bombardment. Callus was selected and regenerated on bialaphos-supplemented medium. All plants were treated with the herbicide Basta six weeks after transfer to the glasshouse and were assayed for herbicide tolerance ten days later. The transgenic natue of the resistant plants were determined by PCR and Southern hybridization. Northern blot analysis confirmed the presence of the bar gene transcript. | |
| sugarcane genetic engineering varieties variety M 292/70 variety M 3035/66 Herbicide Basta herbicide tolerance bar gene embryogenic callus microprojectile bombardment | |
| Mauritius | |
| Cane breeding and genetic improvement | |
| Genetic techniques and protocols | |
| 2009-03-26 | |
| En | |
| LIB | |
| CAT | |
| 1 | |
| BIOTECH |